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Alphabetical Index
Flow Cytometry Crossmatch (8000101630(Flow Cytometry Crossmatch); 8000101631 (Flow Cytometry Crossmatch Deceased))
Test Mnemonic:

FCXM, Flow XM, DD XM
Specimen Requirements:
Collection:

Blood
Container:

FROM RECIPIENT: 2x Pale Yellow (ACD) tube AND 1x Red top tube (no additive)

FROM LIVING DONOR (if applicable): 5x Pale Yellow (ACD) tube AND 1x Red top tube (no additive)

FROM DECEASED DONOR (if applicable): 5x Pale Yellow (ACD) tube AND 1x Red top tube (no additive). Spleen segment or lymph nodes may be used in place of deceased donor blood.
Minimum Volume:

7mL (each tube)
Storage/Transport:

Room temperature, do not refrigerate.
Stability:

Deliver immediately to laboratory.
Causes for Rejection:

Incorrect tube, incorrect label, insufficient white cell count, poor viability.
Methodology:

Flow Cytometry Crossmatch, T-Cell and B-Cell
Performed:

Assessment of donor/recipeint compatibility by flow cytometry crossmatch between donor cells and recipient serum.
Lab:

Tissue Antigen Laboratory
Turnaround Time:

STAT: 8hours, Routine: 5 days
Reference Range:

N/A
CPT 4 Code:

86825 (x2): T-Cell, B-Cell
Note:

FOR DECEASED DONOR FLOW CROSSMATCH, Notify Tissue Antigen Lab On-Call Technologist and/or On-Call Transplant Coordinator immediately after recipient blood draw.
Synonyms:

Flow Crossmatch, Deceased Donor Crossmatch, Flow XM, 
Patient Preparation :

predialysis (if applicable)
Clinical Indication:

Transplant candidate.
Effective Date: 03/08/2023
Reviewed By/Date: -
Approved By/Date: Zou, Jun - 03/07/2023
When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.

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