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Alphabetical Index
Hypercoagulable Evaluation Panel (LT BLUE [LAB002025]; SST [LAB002031]; LAV [LAB002027])
Test Mnemonic:

HYPERCOAGULABLE EVALUATION-LT BLUE

HYPERCOAGULABLE EVALUATION-LT SST

HYPERCOAGULABLE EVALUATION-LT LAV
Specimen Requirements:
Test Included:

PT

APTT

Antithrombin Activity

Protein C Activity

Protein S Free

Antiphospholipid Antibody Evaluation

PT 20210 mutation (send out)

FV Leiden mutation (send out)

 
Collection:

Routine venipuncture; discard 1st mL of blood by collecting a discard tube prior to collecting the blue-topped (3.2% sodium-citrate) tube.  For collections with butterfly blood collection sets, a discard tube should also be collected prior to collection of the blue top to ensure sufficient sample volume. Drawing a discard tube will displace the air from the blood collection set tubing to ensure proper blood draw volume. 
Container:

Blue-topped (3.2% sodium-citrate) tube

Lavender tube

SST tube

 

 
Minimum Volume:

Blue-topped (3.2% sodium-citrate) tube: 5 mL of Plasma

Lavender tube: 3mL WB

SST tube: 1 mL of Serum

 
Storage/Transport:

Blue-topped (3.2% sodium-citrate) tube: 5 mL of Plasma

Shipping/Handling instructions:                                                                           

  1. If delivery time is to be greater than 2 hours from time of draw, centrifuge the specimen for 15 minutes at 2000-2500g.
  2. Transfer plasma to 12x75 plastic tube.
  3. Cap tube and re-spin for 15 minutes at 2000-2500g.
  4. Remove from centrifuge without disturbing any platelet and/or cell pellets that might be on the bottom or sides of the tube.
  5. Carefully transfer plasma into freezer tubes (plastic screw-top cryo-tubes) for testing or freezing and be sure to tape the patient's label onto the tube.
  6. Store transport tube in freezer (-20, or -70C) in an upright position.

Lavender tube: 3mL WB

Transport whole blood at room temperature.

SST tube: 1 mL of Serum

Separate serum from cells within 2 hours of collection: Avoid repeated freeze/thaw cycles.

 
Stability:

Blue-topped (3.2% sodium-citrate) tube: 5 mL of Plasma

Ambient - 2 hours; Refrigerated - Unacceptable; Frozen -20oC – 2 weeks, at -70oC - 6 months 

                                                                                                                                  If the testing is not completed within 2 hours, platelet-poor plasma should be removed without disturbing the sedimented cells (buff-coat) and frozen at -20oC or below for short-term storage (up to 2 weeks), or -70oC for 6 months.

For send out tests refer to specific tests
Causes for Rejection:
  • Clotted and hemolyzed plasma samples are unacceptable and must be redrawn.
  • Wrong tube or anticoagulant.
  • Wrong anticoagulant ratio (over or underfilled tubes).
  • Not centrifuged within the acceptable time after collection.
  • Whole blood or plasma refrigerated or placed on ice prior to testing.
  • Unlabeled.
  • Mislabeled.
Methodology:

Refer to specific test methodology
Performed:

Samples are accepted 24 hours per day at Sample Management, 7.412 CSW Bldg.
Lab:

HYPERCOAGULABLE EVALUATION-LT BLUE - CLS Specialty Lab

Click to view CLS Specialty Lab website 

 

HYPERCOAGULABLE EVALUATION-LT SST - Send out 

 

HYPERCOAGULABLE EVALUATION-LT LAV - Send out
Turnaround Time:

 

The hours of operation are Monday – Friday from 8:00 am to 4:00 pm with an expected TAT of 4 hours. STAT requests after business hours will be evaluated and handled by the on-call staff member.

 
Reference Range:

Refer to specific test reference range 
CPT 4 Code:

Refer to specific test CPT code
Note:

For accurate results Protein C and S assays require the patient to be off warfarin (Coumadin) for at least 2 weeks. Antitrhombin assays requires the patient to be off Low molecular weight heparin (LMWH) and Unfractionated heparin for at least 1 week.

 
Synonyms:

HYPERCOAGULABLE TESTS AND EVALUATION 
Effective Date: 05/29/2019
Reviewed By/Date: Zahner, Christopher J. - 07/08/2019
Approved By/Date: Laposata, Michael - 01/29/2024
When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.

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