This assay is designed to detect mutations in exons 9, 11, 13, 14, 17, and 18 of KIT gene and in exons 12, 14, and 18 of PDGFRA gene. Mutations in KIT and PDGFRA genes may indicate responsiveness to certain targeted therapies. For specific treatment recommendations please refer to clinical practice guidelines, eg, NCCN Guidelines for gastrointestinal stromal tumors (GIST) and melanoma.