Serum separator tube (SST) or Red Top serum tube with no additive 

 Serum separator tube (SST) or Red Top serum tube with no additive 

 3 mL of blood (1mL of serum) 

Refrigerate (2-8°C) up to 7 days; Samples should be frozen (<-20°C)

Refrigerate (2-8°C) up to 7 days; Samples should be frozen (<-20°C)

Gross hemolysis, lipemia, bacterial contamination, improper collection of sample. Specimen container unlabeled or labeled incorrectly. No date and time of collection or no collector information on order.

Within two hours of collection, centrifuge. Serum collected in a red top should be removed from the red cells if testing will be delayed. 

Multiplex Bead Immunoassay

EBV-NA, EBV-EA and EBV VCA can be tested simultaneously with the multiplex assay.  If results are equivocal, a second sample should be collected and tested.

Clinical Microbiology

Test is performed in batch, once per day, six days a week 

86665

Infection by EBV results in the production of antibodies to four different and distinct antigenic complexes: EBV induced Nuclear Antigen (NA), Early Antigen (EA), Viral Capsid Antigen (VCA), and Membrane Antigen. Because of the complex relationship that exists between the EBV/host reaction and clinical manifestation, tracking of EBV antibody patterns may assist in diagnosis of EBV infection. Antibody response profiles for the different EBV antigens demonstrate a characteristic pattern for silent primary or persistent latent EBV infection.

Previously positive patients do not require repeat testing.

Epstein Barr Virus Antibody, IgG; EBV antibody, IgG; EBV IgG antibody; EBV Viral Capsid Antibody, IgG; EBVG

Routine venipuncture

IgG antibodies to VCA are normally present during acute and convalescent EBV disease. Antibody levels tend to rise and peak after 3-4 weeks, then decline and usually persist for life.  Order in conjunction with VCA IgM antibodies and anti-EBNA antibodies to differentiate acute, recent, and past EBV infection.