Syphilis Total. Reactive test results are automatically tested for RPR titer and TP-PA confirmation tests, as needed.

Serum separator tube (SST) or Red top serum tube with no additive

Serum separator tube (SST) or Red top serum tube with no additive

3 mL of blood (1 ml of serum)

Refrigerate (2-8°C) up to 7 days; Samples should be frozen (<-20°C)

Refrigerate (2-8°C) up to 7 days; Samples should be frozen (<-20°C)

Insufficient quantity, gross hemolysis, lipemia.  Specimen container unlabeled or labeled incorrectly. No date and time of collection or collector completed.

Within two hours of collection, centrifuge. Serum collected in a red top tube should be removed from the red cells if testing will be delayed.

Multiplex immunoassay

Clinical Microbiology

Test is performed in batch, once per day, six days a week

L&D/Newborn specimens tested 7 days a week.

Non-reactive

86780

Specimens with positive Syphilis Total results are automatically tested for RPR titer and a confirmatory treponemal test (Treponema pallidum partical agglutination test), as needed.

For patients diagnosed with syphilis (i.e., positive Total and confirmatory test), the CDC recommends that response to therapy be assessed by RPR test (a 4-fold decline in titer, or undetectable RPR titer) after 6 months. 

Tests are performed on all obstetrics patients regardless of previous results.  If there is no previous patient data, the requested tests are performed.

VDRL test is performed on CSF samples as a Sendout test for patients with positive serum test results, only.

 Syphilis

Routine venipuncture

Intended for the qualitative detection of total antibodies to Treponema pallidium and should be used in conjunction with other laboratory tests and clinical findings to aid in the diagnosis of syphilis infection.